Preparative separation and identification of novel subsidiary colors of the color additive D&C Red No. 33 (Acid Red 33) using spiral high-speed counter-current chromatography
نویسندگان
چکیده
منابع مشابه
Preparative Separation of Polyphenols from Artichoke by Polyamide Column Chromatography and High-speed Counter-current Chromatography
An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples w...
متن کاملPreparative separation of isovitexin and isoorientin from Patrinia villosa Juss by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v) was used to isolate and separate two C-glycosylflavones from Patrinia villosa Juss, a traditional Chinese medicine. The separation produced 42.9 mg isovitexin and 20.1 mg isoorientin with purities of 99.3% and 98.5%, respectively as determined by high-performance liqu...
متن کاملPreparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography
A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR. This method was simple, fast, convenient and appropriate to prepare pure compound as reference su...
متن کاملSample capacity in preparative high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) is a versatile technique in preparative separation and purification of pure compounds from complex matrices. As a preparative chromatography, there is a need to maximize the column production. Based on the plate theory of Van Deemter, the effect of the sample load on the separation was investigated in a preparative HSCCC with a 1000 ml column ca...
متن کاملApplication of High-Speed Counter-Current Chromatography Preparative Separation of Flavone C-Glycosides From Lophatherum gracile
Application of High-Speed Counter-Current Chromatography Preparative Separation of Flavone CGlycosides From Lophatherum gracile Qingfa Tang c , Ying Wang a b , Mei Chen a b , Qingwen Zhang d , Chunlin Fan a b , Xiaojun Huang a b , Yaolan Li a b & Wencai Ye a b a Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University , Guangzhou , P. R. China b Guangd...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2015
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2014.12.072